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Discovery Antibodies/Stichodactyla Toxin-Biotin/5 x 5 µg/STS-400-B-5 x 5 µg,Discovery,,Discovery Antibodies,discoveryantibodies,Discovery,Discovery,+,,Array.Array.Array蚂蚁淘厂家直采.
主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Discovery Antibodies/Stichodactyla Toxin-Biotin/5 x 5 µg/STS-400-B-5 x 5 µg
产品编号:STS-400-B-5x5µg
市  场 价:¥29680.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗地高辛>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1484.00
品      牌: Discovery
公      司:Discovery Antibodies
公司分类:
Discovery Antibodies/Stichodactyla Toxin-Biotin/5 x 5 µg/STS-400-B-5 x 5 µg
商品介绍
Cat#:STS-400-B
AlternativeNameK+ channeltoxinShK, Toxinκ-stichotoxin-She1a,κ-SHTX-She1a
  • LyophilizedPowder
  • BioassayTested
  • OriginSyntheticpeptide
    MW:4563Da.
    Purity:>98%(HPLC)
    FormLyophilizedpowder.
    Effectiveconcentration1-100nM.
    SequenceRSCIDTIPKSRCTAFQCKHSMKYRLSFCRKTCGTC.
    ModificationsDisulfidebondsbetweenCys3-Cys35,Cys12-Cys28,andCys17-Cys32.
    LabelLC-Biotin.Theextentoflabelingis1moleculeofbiotinpermoleculeofShK.
      • Stichodactyla Toxin-Biotin
    ActivityStichodactylaToxinblocksKV1.3,KV1.1,KV1.4,andKV1.6atsubnanomolarconcentrationsandKV3.2channelsat1000-foldhigherconcentrationthanthatrequiredtoinhibitKV1.3channels1-3.
      • Pennington,M.W. etal.(1995) Int.J.Pept.ProteinRes. 46,354.
      • Beeton,C. etal.(2003) J.Biol.Chem. 278,9928.
      • Middleton,R.E. etal.(2003) Biochemistry 42,13698.
    ShippingandstorageShippedatroomtemperature.Productassuppliedcanbestoredintactatroomtemperatureforseveralweeks.Forlongerperiods,itshouldbestoredat-20°C.
    SolubilityAnyaqueousbuffer.Centrifugeallproductpreparationsbeforeuse(10000xg5min).
    StorageofsolutionsUptotwoweeksat4°Corthreemonthsat-20°C.
      • Stichodactyla Toxin-Biotin
        AlomoneLabsStichodactylaToxin-Biotinstainsratcerebellum.
        Mildlyfixedratcerebellumsectionswereincubatedwith25nMStichodactylaToxin-Biotin(#STS-400-B)followedbystreptavidin-Cy3(red).StainingofPurkinjecells(verticalarrows)isseenacquiredbyA.25xobjectiveB.40xobjective. Usingthe40xobjective,grainsscatteredoverthesomaofpurkinjecellscanbeseen(horizontalarrows).DAPI(blue)isusedasthecounterstain.
        Stichodactyla Toxin-Biotin
        AlomoneLabsStichodactylaToxin-BiotinblocksKV1.3channelsexpressedin Xenopus oocytes.
        A.Representativetimecourseof StichodactylaToxin-Biotin(#STS-400-B)inhibitionofKV1.3current.Membranepotentialwasheldat-100mV,currentwaselicitedbya100msvoltagerampto+60mVevery10sec,andsignificantlyinhibitedby1nMStichodactylaToxin-Biotin(green).B.SuperimposedtracesofKV1.3currentfollowingapplicationofcontrol(black)andof1nMStichodactylaToxin-Biotin(green),takenfromtherecordinginA.
    References-Scientificbackground
    • 1.Castaneda,O. etal.(1995) Toxicon 33,603.
    • 2.Pennington,M.W. etal.(1995) Int.J.Pept.ProteinRes. 46,354.
    • 3.Beeton,C. etal.(2003) J.Biol.Chem. 278,9928.
    • 4.Middleton,R.E. etal.(2003) Biochemistry 42,13698.
    • 5.Yan,L. etal.(2005) Mol.Pharmacol. 67,1513.
      • ShK(StichodactylaToxin)isapeptidyltoxinoriginallyisolatedfromthenematocystoftheseaanemone Stichodactylahelianthus.1

        ShKblocks KV1.3, KV1.1, KV1.4,and KV1.6 atsubnanomolarconcentrationsandKV3.2channelsat1000-foldhigherconcentrationthanthatrequiredtoinhibitKV1.3channels.IthasbeenshowntoblockKV currentinDRGneurons,todisplacerADIoactiveDendrotoxinfrombrainsynaptosomesandinhibitI125-CharyBDotoxinbindingtoJurkatTlymphocyteswithanIC50 of32pM.2,4

        EvidencealsosuggeststhatnativeKV currentsinthecentralnervoussystem,whicharepredominantlycarriedby KV1.2 channels,arehighlysensitivetothistoxin.5

        AfluorescentlylabeledsyntheticShKisusedtorecognizeandtosortKV1.3containinglymphocytes.4,6

    TargetKV1.1,KV1.3,KV1.4,KV1.6channels
    NetPeptideContent:100%
    Lastupdate:13/11/2019

    StichodactylaToxin-Biotin (#STS-400-B) isahighlypure,synthetic,andbiologicallyactivepeptidetoxinconjugatedtobiotin.

    BenefitsofStichodactylaToxin-Biotin:
     Localizationanddistribution
     Clusteringandinternalizationkinetics
     Livecellimaging
     Singlecelldetection
     Bindingkinetics

    Wegladlytakeoncollaborationprojects.PleaseContactUs.

    Forresearchpurposesonly,notforhumanuse
    品牌介绍

    Discoveryantibodies


    CRB是世界上第二大肽公司,也是抗体研究市场的先驱,它现在正在探索抗体的旗帜下建立一批新验证的目标抗体。


    CRB, the 2nd oldest peptide company in the world and forerunner in the antibody research market is now building a collection of newly validated target antibodies under the banner of Discovery® Antibodies.


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