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Discovery Antibodies/Anti-KV1.3 (KCNA3) Antibody/50 µl/APC-002-50 µl,Discovery,,Discovery Antibodies,discoveryantibodies,Discovery,Discovery,+,,Array.Array.Array蚂蚁淘厂家直采.
主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Discovery Antibodies/Anti-KV1.3 (KCNA3) Antibody/50 µl/APC-002-50 µl
产品编号:APC-002-50µl
市  场 价:¥7340.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>其他二抗>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$367.00
品      牌: Discovery
公      司:Discovery Antibodies
公司分类:
Discovery Antibodies/Anti-KV1.3 (KCNA3) Antibody/50 µl/APC-002-50 µl
商品介绍
Cat#:APC-002
AlternativeNamePotassiumvoltage-gatedchannelsubfamilyAmember3
  • LyophilizedPowder
  • AntigenIncl.
  • Type:Polyclonal
    Host:Rabbit
    Reactivity:h,m,r
      • GSTfusionproteinwiththesequenceTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV,correspondingtoaminoacidresidues523-575ofhumanKV1.3(Accession P22001).Intracellular,C-terminus.
        Anti-KV1.3 (KCNA3) Antibody
    Accession(Uniprot)NumberP22001
    GeneID3738
    PeptideconfirmationConfirmedbyDNAsequenceandSDS-PAGE.
    HomologyRat,rabbit,mouse-identical.
    RRIDAB_2040151.
    PurityTheserumwasdepletedofanti-GSTantibodiesbyaffinitychromatographyonimmobilizedGSTandthentheIgGfractionwaspurifiedonimmobilizedantigen.
    FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,5%sucrose,0.025%NaN3.
    IsotypeRabbitIgG.
    StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
    Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
    Antibodyconcentrationafterreconstitution0.6mg/ml.
    StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
    NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
    Negativecontrolantigenreconstitution100μlPBS.
    Negativecontrolantigenstorageafterreconstitution-20°C.
    PreadsorptionControl3µgfusionproteinper1µgantibody.
    StandardqualitycontrolofeachlotWesternblotanalysis.
    Applications:ic,if,ih,ip,wb
    Mayalsoworkin:ifc*
      • Anti-KV1.3 (KCNA3) Antibody
        Westernblotanalysis ofratbrainmembranes:
        1. Anti-KV1.3 (KCNA3) Antibody(#APC-002),(1:200).
        2.Anti-KV1.3(KCNA3)Antibody,preincubatedwiththenegativecontrolantigen.
        HumanCD3+cells(Szigligeti,P. etal. (2006) J.Physiol. 573, 357.).
      • TransfectedHEK-293celllysate(Vicente,R. etal. (2006) J.Biol.Chem. 281, 37675.).
      • Ratbrainsections.
      • RatneuralProgenitorcells(NPCs)(1:200)(Liebau,S. etal. (2006) J.NeuRochem99, 426.).
    References
    • 1.Chandy,K.G.etal.(2001)Toxicon39,1269.
    • 2.Koo,G.C.etal.(1997)J.Immunol.158,5120.
    • 3.Grissmer,S.etal.(1994)Mol.Pharmacol.45,1227.
    • 4.Garcia-Calvo,M.etal.(1993)J.Biol.Chem.268,18866.
    • 5.Garcia,M.L.etal.(1994)Biochemistry33,6834.
    • 6.Koschak,A.etal.(1998)J.Biol.Chem.273,2639.
    • 7.Kalman,K.etal.(1998)J.Biol.Chem.273,32697.
      • KV1.3belongstothe Shaker familyof voltage-dependentK+ channels. Thechannel,encodedby KCNA3,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV1.3isthemainchannelresponsIBLeformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1

        KV1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBDotoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM) and Stichodactylatoxin (0.01nM).3-7

    Applicationkey:

    CBE-Cell-basedELISA,FC-Flowcytometry,ICC-Immunocytochemistry,IE-IndirectELISA,IF-Immunofluorescence,IFC-Indirectflowcytometry,IHC-Immunohistochemistry,IP-Immunoprecipitation,LCI-Livecellimaging,N-Neutralization,WB-Westernblot

    Speciesreactivitykey:

    H-Human,M-Mouse,R-Rat
    receivea25µlfreetrialsample!
    Lastupdate:08/11/2019

    AlomoneLabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofhumanKV1.3.Anti-KV1.3(KCNA3)Antibody(#APC-002)canbeusedinwesternblot,immunoprecipitation,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeKV1.3potassiumchannelfromhuman,rat,andmousesamples.

    Forresearchpurposesonly,notforhumanuse
    品牌介绍

    Discoveryantibodies


    CRB是世界上第二大肽公司,也是抗体研究市场的先驱,它现在正在探索抗体的旗帜下建立一批新验证的目标抗体。


    CRB, the 2nd oldest peptide company in the world and forerunner in the antibody research market is now building a collection of newly validated target antibodies under the banner of Discovery® Antibodies.


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