AlternativeName Potassiumvoltage-gatedchannelsubfamilyAmember3
LyophilizedPowder LyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl. AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type: Polyclonal
Host: Rabbit
Reactivity: h,m,r
Immunogen- + GSTfusionproteinwiththesequenceTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV,correspondingtoaminoacidresidues523-575ofhumanKV 1.3(Accession P22001).Intracellular,C-terminus.
Accession(Uniprot)Number P22001
GeneID 3738
Peptideconfirmation ConfirmedbyDNAsequenceandSDS-PAGE.
Homology Rat,rabbit,mouse-identical.
RRID AB_2040151.
Purity Theserumwasdepletedofanti-GSTantibodiesbyaffinitychromatographyonimmobilizedGSTandthentheIgGfractionwaspurifiedonimmobilizedantigen.
Form Lyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,5%sucrose,0.025%NaN3 .
Isotype RabbitIgG.
Storagebeforereconstitution Theantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution 25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution 0.6mg/ml.
Storageafterreconstitution Thereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
Negativecontrolantigenstoragebeforereconstitution Lyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution 100μlPBS.
Negativecontrolantigenstorageafterreconstitution -20°C.
PreadsorptionControl 3µgfusionproteinper1µgantibody.
Standardqualitycontrolofeachlot Westernblotanalysis.
Applications: ic,if,ih,ip,wb
Mayalsoworkin: ifc*
Westernblot- + Westernblotanalysis ofratbrainmembranes:
1. Anti-KV 1.3 (KCNA3) Antibody (#APC-002),(1:200). 2.Anti-KV 1.3(KCNA3)Antibody,preincubatedwiththenegativecontrolantigen.
HumanCD3+cells(Szigligeti,P. etal. (2006) J.Physiol. 573, 357.).
Immunoprecipitation- + TransfectedHEK-293celllysate(Vicente,R. etal. (2006) J.Biol.Chem. 281, 37675.).
Immunocytochemistry- + Ratneural
Progen itorcells(NPCs)(1:200)(Liebau,S.
etal. (2006)
J.NeuRoche m .
99, 426.).
References
1.Chandy,K.G.etal. (2001)Toxicon 39, 1269. 2.Koo,G.C.etal. (1997)J.Immunol. 158, 5120. 3.Grissmer,S.etal. (1994)Mol.Pharmacol. 45, 1227. 4.Garcia-Calvo,M.etal. (1993)J.Biol.Chem. 268, 18866. 5.Garcia,M.L.etal. (1994)Biochemistry 33, 6834. 6.Koschak,A.etal. (1998)J.Biol.Chem. 273, 2639. 7.Kalman,K.etal. (1998)J.Biol.Chem. 273, 32697. Scientificbackground- + KV 1.3belongstothe Shaker familyof voltage-dependentK+ channels. Thechannel,encodedby KCNA3 ,iswidelyexpressedinthebrain,lungandosteoclastsandinseveralcell populationsofhematopoieticorigin.TheprominenceofKV 1.3channelsinthesecells(particularlyinTlymphocytes)directedmuchresearch attention.ItwasfoundthatKV 1.3isthemainchannelresponsIBL eformaintainingtherestingpotentialinquiescentcellsandregulatingthe Ca2+ signalingthatisindispensablefornormalTlymphocyteactivation.1,2 BasedonthecentralroleofKV 1.3inregulatingtheinitiationofanimmuneresponse, thechannelhasbeenrecognizedasapotentialtargetforimmunosuppressantdrugs.1
KV 1.3channelsarepotentlyinhibitedbyseveralvenomouspeptidetoxinsamongthem CharyBD otoxin (2.6nM), Noxiustoxin (1nM), Kaliotoxin (0.65nM), Margatoxin (0.05nM), Agitoxin-1 (1.7nM), Agitoxin-2 (0.004nM), Hongotoxin-1 (0.09nM) and Stichodactylatoxin (0.01nM).3-7
LyophilizedPowder NegativeControlAntigenIncluded
Clickhereto receivea25µl freetrialsample!
Lastupdate:08/11/2019
Alomone LabsispleasedtoofferahighlyspecificantibodydirectedagainstanepitopeofhumanKV 1.3.Anti-KV 1.3(KCNA3)Antibody (#APC-002)canbeusedinwesternblot,immunoprecipitation,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeKV 1.3potassiumchannelfromhuman,rat,andmousesamples.
Forresearchpurposesonly,notforhumanuse