Anti-CACNA1G(CaV3.1)Antibody(#ACC-021)isahighlyspecificantibodydirectedagainstanepitopeoftheratCaV3.1channel.Theantibody canbeusedinwesternblot,immunocytochemistry,andimmunohistochemistryapplications.IthasbeendesignedtorecognizeCACNA1G fromhuman,rat,andmousesamples.
产品编号：ACC-021-25µl
市 场 价：¥4940.00
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商品介绍
AlternativeNameVoltage-dependentT-typecalciumchannelsubunitα1G
KOValidatedKOValidated
Thespecificityofthisantibodyhasbeenvalidatedinaknockout(KO)orknockdown(KD)system.
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type:Polyclonal
Host:Rabbit
Reactivity:h,m,r
- PeptideMDEEEDGAGAEESGQPRSFTQL(C),correspondingtoaminoacidresidues1-22ofratCACNA1G (Accession O54898).Intracellular,N-terminus.
Accession(Uniprot)NumberO54898
GeneIDO54898
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse-identical;human-20/22aminoacidresiduesidentical.
RRIDAB_2039779.
PurityAffinitypurifiedonimmobilizedantigen.
FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
IsotypeRabbitIgG.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50µlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution-20°C.
PreadsorptionControl1µgpeptideper1µgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,if,ih,wb
Mayalsoworkin:ifc*,ip*
Westernblotanalysisofratbrainmembranes:1. Anti-CACNA1G(CaV3.1) Antibody(#ACC-021),(1:200).
2.Anti-CACNA1G(CaV3.1)Antibody,preincubatedwiththenegativecontrolantigen.
ExpressionofCACNA1G inratcerebellumImmunohistochemicalstainingofratcerebellumusing Anti-CACNA1G(CaV3.1) Antibody(#ACC-021).A.CACNA1G immunoreactivity(green)appearsinthemolecularlayer.B.NuclearstainingusingDAPIasthecounterstain(blue).C.MergedimagesAandB.Mol=molecularlayer.Humanmyometrium(1:200)(Blanks,A.M. etal. (2007) J.Physiol. 581, 915.).
- Humanmyometriumcells(1:200)(Blanks,A.M. etal. (2007) J.Physiol. 581, 915.).
References
- 1.Serrano,J.R.etal.(1999)J.Gen.Physiol.114,185.
- 2.Perez-Reyes,E.(2003)Physiol.Rev.83,117.
- 3.Wain,H.M.etal.(2002)NucleicAcidsRes.30,169.
- 4.Andreasen,D.etal.(2000)Am.J.Physiol.279,F997.
Voltage-dependentCa2+ channelsprovideapathwayforrapidinfluxofCa2+ intocells, whichplaysacrucialroleinbothelectricalandmetabolicsignaling.1
T-typecurrentsaretransducedviachannelproteinsencodedbythreegenesthat composeasubfamilywithintheCaVchannelfamily.2-3
TheactivityofT-typechannelscontributestoseveralknownphysiologicalandpathophysiological phenomenaincludingburstfiringinneurons,pacemakingactivityintheheartandsecretionfromendocrinetissues.2 TherearethreeclonedT-typechannelisoforms.
CACNA1G(CaV3.1)andCACNA1H(CaV3.2)arewidelydistributedwhereastheexpressionofCACNA1I(CaV3.3) isrestrictedtothecentralnervoussystem.2
CACNA1GandCACNA1H arealsoexpressedinthekidney,butlittleisknownabouttheirphysiologicalrolethere.
LyophilizedPowderNegativeControlAntigenIncluded
Image&Title:
ExpressionofCaV3.1inmousepelviskidneyjunction.
Immunohistochemicalstainingofmousekidneysectionsusing Anti-CACNA1G(CaV3.1)Antibody (#ACC-021).A.CaV3.1(purple)isdetectedinthepelviskidneyjunction(PKJ).B.HCN3staining(green),aMarkerofpacemakercells,isdetectedinthePKJ.C.SamesectionstainedforCaV3.1(red).D.MergeofBandCpanelsshowshighdegreeofco-localizationbetweenCaV3.1andHCN3.
Adaptedfrom Hurtado,R.etal. (2014)withpermissionof FederationofAmericanSocietiesforExperimentalBIOLOGy.
ExpressionofCaV3.1inmousepelviskidneyjunction.
Immunohistochemicalstainingofmousekidneysectionsusing Anti-CACNA1G(CaV3.1)Antibody (#ACC-021).A.CaV3.1(purple)isdetectedinthepelviskidneyjunction(PKJ).B.HCN3staining(green),aMarkerofpacemakercells,isdetectedinthePKJ.C.SamesectionstainedforCaV3.1(red).D.MergeofBandCpanelsshowshighdegreeofco-localizationbetweenCaV3.1andHCN3.
Adaptedfrom Hurtado,R.etal. (2014)withpermissionof FederationofAmericanSocietiesforExperimentalBIOLOGy.
Lastupdate:10/11/2019
Forresearchpurposesonly,notforhumanuse
品牌介绍
Discoveryantibodies
CRB是世界上第二大肽公司，也是抗体研究市场的先驱，它现在正在探索抗体的旗帜下建立一批新验证的目标抗体。
CRB, the 2nd oldest peptide company in the world and forerunner in the antibody research market is now building a collection of newly validated target antibodies under the banner of Discovery® Antibodies.
品牌 | 货号 | 名称 | 规格 |
Discoveryantibodies | crb2005253 | Anti-crc antibody | 20ul 100ul |
Discoveryantibodies | crb2005041 | Anti-IL-11 antibody | 20ug 100ug |
Discoveryantibodies | crb2005038 | Anti-RAMP1 antibody | 20ug 100ug |
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