This product is freeze dried. All water molecules have been removed.
Antigen Incl.
Antigen Incl.
This antibody is shipped with its antigen FREE of charge!
Type: Polyclonal
Host: Guinea Pig
Reactivity: h, m, r
Peptide (C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor (Accession P12526). 3rd intracellular loop.
Accession (Uniprot) Number P12526
Gene ID 25153
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Negative control antigen reconstitution 100 µl double distilled water (DDW).
Negative control antigen storage after reconstitution -20°C.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1,2. Guinea pig Anti-Angiotensin-(1-7) Mas ReceptorAntibody (#AGP-099), (1:600). 3,4. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody, preincubated with the negative control antigen.
References
1. Santos, R.A. et al. (2003) Proc. Natl. Acad. Sci. U.S.A.100, 8258.
2. Alenina, N. et al. (2008) Exp. Physiol.93, 528.
3. Santos, R.A. et al. (2008) Exp. Physiol.93, 519.
The Angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide angiotensin-(1-7).1
Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis.
Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3
The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene.
Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3
The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry,IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation,LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
Lyophilized PowderNegative Control Antigen Included
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Certificate of Analysis MSDS
Last update: 14/08/2019
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat protein. Guinea pig Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AGP-099) raised in guinea pig can be used in western blot analysis. It has been designed to recognize Ang-(1-7) Mas receptor from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-Angiotensin-(1-7) Mas Receptor Antibody (#AAR-013) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.
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