Anti-KCNN2(KCa2.2,SK2)Antibody(#APC-028)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunocytochemistry,immunohistochemistry,andimmunoprecipitationapplications.IthasbeendesignedtorecognizeKCNN2fromhuman,rat,andmousesamples.
产品编号：APC-028-50µl
市 场 价：¥7340.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
邮 箱：
info@ebiomall.com
商品介绍
AlternativeNameSKCa2, Smallconductancecalcium-activatedpotassiumchannelprotein2, Apamin-sensitivesmallconductanceCa2+-dependentK+channel
LyophilizedPowderLyophilizedPowder
Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
AntigenIncl.AntigenIncl.
ThisantibodyisshippedwithitsantigenFREEofcharge!
Type:Polyclonal
Host:Rabbit
Reactivity:h,m,r
- Peptide(C)ETQMENYDKHVTYNAERS,correspondingtoaminoacidresidues542-559ofratKCNN2 (Accession P70604).Intracellular,C-terminalpart.
Accession(Uniprot)NumberP70604
GeneID54262
PeptideconfirmationConfirmedbyaminoacidanalysisandmassspectrometry.
HomologyMouse-17/18aminoacidresiduesidentical;human-16/18aminoacidresiduesidentical;chicken-14/18aminoacidresiduesidentical.
RRIDAB_2040126.
PurityAffinitypurifiedonimmobilizedantigen.
FormLyophilizedpowder.Reconstitutedantibodycontainsphosphatebufferedsaline(PBS),pH7.4,1%BSA,0.05%NaN3.
IsotypeRabbitIgG.
StoragebeforereconstitutionTheantibodyshipsasalyophilizedpowderatroomtemperature.Uponarrival,itshouldbestoredat-20°C.
Reconstitution25µl,50μlor0.2mldoubledistilledwater(DDW),dependingonthesamplesize.
Antibodyconcentrationafterreconstitution0.8mg/ml.
StorageafterreconstitutionThereconstitutedsolutioncanbestoredat4°Cforupto1week.Forlongerperiods,smallaliquotsshouldbestoredat-20°C.Avoidmultiplefreezingandthawing.Centrifugeallantibodypreparationsbeforeuse(10000xg5min).
NegativecontrolantigenstoragebeforereconstitutionLyophilizedpowdercanbestoredintactatroomtemperaturefor2weeks.Forlongerperiods,itshouldbestoredat-20°C.
Negativecontrolantigenreconstitution100µldoubledistilledwater(DDW).
Negativecontrolantigenstorageafterreconstitution-20°C.
PreadsorptionControl1μgpeptideper1μgantibody.
StandardqualitycontrolofeachlotWesternblotanalysis.
Applications:ic,if,ih,ip,wb
Mayalsoworkin:ifc*
Westernblotanalysis ofratbrainmembranes:1. Anti-KCNN2(KCa2.2,SK2) Antibody#APC-028),(1:200).
2.Anti-KCNN2(KCa2.2,SK2)Antibody,preincubatedwiththenegativecontrolantigen.HumanJurkatTcells(1.5µg/ml)(Desai,R. etal. (2000) J.Biol.Chem. 275,39954.).
Humanandmouseheart(Xu,Y. etal. (2003) J.Biol.Chem. 278, 49085.).
- Rathippocampalslices(2µg/ml)(Kramar,E.A. etal. (2004) J.Neurosci. 24, 5151.).
ExpressionofKCNN2(SK2)inratcerebralcortexImmunohistochemicalstainingofKCNN2 channelsusing Anti-KCNN2(KCa2.2,SK2) Antibody (#APC-028)inratcerebralcortex.Pictureshowingthethethirdlayer.Notethatbothneural(blackarrows)andglialcells(greenarrows)showintensestaining.ReactionproductisredandcounterstainisH&E.
- Ratneuronsfromhippocampus(1:200)(Wang,W. etal. (2011) Neuropharmacology 60, 901.).
References
- 1.Kohler,M.etal.(1996)Science273,1709.
- 2.Xia,X.M.etal.(1998)Nature395,503.
- 3.Stocker,M.(2004)Nat.Rev.Neurosci.5,758.
KCa2.2 (KCNN2,SK2)isamemberoftheCa2+-activatedK+ channelfamilywithsmallconductancethatincludes KCa2.1 (KCNN1,SK1)and KCa2.3 (KCNN3,SK3).ThechannelisvoltageinsensitiveandisactivatedbyintracellularCa2+ inthesubmicromolarrange.Ithas,though,asimilartopologytothatofvoltage-dependentK+ channels(KV channels),thatissixtransmembranedomainsandintracellularN-andC-termini.ThefunctionalchannelofalltheKCa2familymembersisamultimericproteincomposedoffourpore-formingsubunits.
KCa2channelsareextremelysensitivetothelevelsofintracellularCa2+ andconcentrationsaslowas300-700nMcanopenthechannelsveryrapidly(5-15ms).Hence,theKCa2channelsarehighlysensitiveandfastCa2+ sensorsresemblingotherknownCa2+-bindingproteins.ThistypeofCa2+-dependentactivationisachievedbytheconstitutivebindingoftheKCa2channelstocalmodulin,ahighlyexpressedCa2+-bindingproteinviaacalmodulin-bindingdomainsituatedatthecytoplasmicC-terminus.
Pharmacologically,theKCa2channelsaretheonlyknowntargetsofthebeevenomtoxin Apamin,withKCa2.1beingtheleastsensitive,KCa2.2themostsensitiveandKCa2.3showingintermediatesensitivity.
KCa2.2ispredominantlyexpressedinthenervoussystemalthoughexpressioninendothelialcells,heartandliverhasbeendescribed.
KCa2.2isknowntobeinvolvedintheregulationofneuronalexcitABIlity.TheydosomainlyviaaphenomenonknownasafterhyperpolarizationinwhichKCa2channelsopeninresponsetoincreasedintracellularCa2+ concentrationsthatresultfromtheentryofextracellularCa2+ throughvoltage-dependentCa2+ channelsduringactionpotentials.Inthisway,KCa2channelseffectivelyformaCa2+-mediatedfeedbackloop.
LyophilizedPowderNegativeControlAntigenIncluded
Clickheretoreceivea25µlfreetrialsample!
Lastupdate:08/11/2019
Forresearchpurposesonly,notforhumanuse
品牌介绍
Discoveryantibodies
CRB是世界上第二大肽公司，也是抗体研究市场的先驱，它现在正在探索抗体的旗帜下建立一批新验证的目标抗体。
CRB, the 2nd oldest peptide company in the world and forerunner in the antibody research market is now building a collection of newly validated target antibodies under the banner of Discovery® Antibodies.
品牌 | 货号 | 名称 | 规格 |
Discoveryantibodies | crb2005253 | Anti-crc antibody | 20ul 100ul |
Discoveryantibodies | crb2005041 | Anti-IL-11 antibody | 20ug 100ug |
Discoveryantibodies | crb2005038 | Anti-RAMP1 antibody | 20ug 100ug |
公司介绍
产品分类
Calcium Sensing Receptor
Antibody & Peptide Bundles
Polyclonal Antibodies
Adhesion Class GPCRs
Antigen Peptides
Calcitonin Receptors
Antibody & Peptide Bundles
Polyclonal Antibodies
Antigen Peptides
Antibodies A - Z
Antibodies
BAI Receptors
Labelled Antibodies
Monoclonal Antibodies
Products
Products
Adenosine Receptors
Angiotensin II Receptors
Angiotensin-(1-7) Mas Receptor
Cannabinoid Receptors
Labelled Antibodies
Home
Antigen Peptides
销售排行
厂家直采
全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关，提供发票
及时交付
限时必达 不达必赔
总部地址：
江苏省苏州市工业园区星湖街218号 苏州生物医药产业园一期（生物纳米园）A4-216(邮编：215125)
工作时间：周一至周五9:00~18:00
技术支持：
短信：18915418616
服务热线：
4000-520-616（全国）
☏ 0512-67156496（苏州）
版权信息：
© 2015- 苏州蚂蚁淘生物科技有限公司 版权所有 中国
友情链接：
联系QQ:764052152
免责声明：
本站有部分内容来自互联网，如无意中侵犯了某个媒体 、公司 、企业或个人等的知识产权，请来电或致函告之，
本网站将在规定时间内给予删除等相关处理。
本网站将在规定时间内给予删除等相关处理。
扫描二维码关注官方微信
获取最新促销及产品信息